et al., “Charaterization of Hotspots in a Highly Enhancing SERS Substrate,” Analyst, 2011, 136, 4472-4479. Negri, Pierre et al., “Online SERS Detection and Characterization of Eight Biologically-Active Peptides Separated by Capillary Zone Electrophoresis,” Analyst, 2015, 140, 1516-1522.Īsiala, Steven M. (2007), SERS as a Foundation for Nanoscale, Optically Detected Biological Labels. Gas chromatograph, Fourier transform, infrared spectroscopy systemĭoering, W. Surface enhanced raman scattering from metal nanoparticle-analyte-noble metal substrate sandwiches Ribbon flow cytometry apparatus and methods Optical detection and analysis of sub-micron particles Surface-enhanced spectroscopy-active sandwich nanoparticles Surface-enhanced Raman spectroscopy for biosensor systems and methods for determining the presence of biomolecules Multilayer hydrodynamic sheath flow structureĭetermination of the number concentration and particle size distribution of nanoparticles using dark-field microscopy Surface modification of metals for biomolecule detection using surface enhanced Raman scattering (SERS) Microfluidic apparatus, Raman spectroscopy systems, and methods for performing molecular reactions Surface plasmon resonance sensor and sensor chip Nucleic acid analysis device and nucleic acid analyzer using the sameĬoaxial microreactor for particle synthesis The flow detector offers substantial advantages over conventional SERS-based assays such as minimal sample volumes and high detection efficiency. At low analyte concentrations, rapid analyte desorption is observed, enabling repeated and high-throughput SERS detection. Raman experiments at different sheath flow rates showed increased sensitivity compared with the modeling predictions, indicating increased adsorption. Increased analyte interactions with the SERS substrate significantly improve detection sensitivity. The device employs hydrodynamic focusing to improve SERS detection in a flow channel where a sheath flow confines analyte molecules eluted from a capillary over a planar SERS-active substrate. A surface-enhanced Raman scattering (SERS) flow detector capable of ultrasensitive optical detection on the millisecond time scale has been developed. The invention provides an apparatus and methods for label-free, chemical specific detection in flow for high throughput characterization of analytes in applications such as flow injection analysis, electrophoresis, and chromatography.